I am working with primary human fibroblasts from patients with severe metabolic disease. I am interested in using RNA/mRNA re-programming of these fibroblasts in order to obtain iPSCs for downstream functional characterisation.
In recent years, numerous kits/protocols have cropped up purporting to tackle this issue. I was wondering if you could share your experience of this approach and which method you found most effective?
Many thanks,
Rob.
RNA-based Reprogramming of Human Primary Fibroblasts into Induced which is not fixed to develop it any ways by the Stem Cells
I recommend Stemgent® StemRNA™ 3rd Gen Reprogramming Kit for hiPSC generation from human skin fibroblast. In our lab, the RNA-based reprogramming has a higher efficiency than Sendai virus based and plasmid based methods, especially when the start cells were skin fibroblasts.
ps: the recommended hypoxia condition in kit's instructions is not needed. Typical iPSC colonies can be picked 14-20 days post 4-round-RNA transfection
@. Robert Flynn
Good luck.
Hi Jing Liu . That sounds very promising. I will definitely check it out. Thanks for your answer!
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