What are the procedures for environment friendly and effective methods to prepare silicon QDs, Si nanotubes and silicene (2D materials)?

There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...

10 January 2021 3,418 1 View

What are the methods to eliminate or minimize total dissolve solids in industrial effluent ?

technology relevant methods or processes

02 January 2021 6,632 2 View

Does the adsorbed water or gases affect the CHNS analysis of high surface area porous carbon ?

Adsorbed water and gases can affect the weight of the porous carbon. Some times 6-7% weight loss can occur during the degassing of the sample. Will this weight loss affect the calculation of C,...

08 November 2020 5,399 3 View

What is RNA filtration technique?

Sir, How I Can filter the only RNA as well as DNA separately from the whole genome after lysing using a membrane, and which membrane filter is suitable for the same.

14 September 2020 601 1 View

How to solve this error in a Heat Exchange Reformer model in Aspen Custom Modeler?

I have written code for a for a Heat Exchange Reformer in Aspen Custom Modeler, but it cannot run. The program describes a shell and tube heat exchanger with steam methane reforming reactions...

07 September 2020 2,024 9 View

Why is that only vaccine is available for Hepatitis B and not a drug that can eradicate it?

Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...

22 June 2020 5,661 3 View

What is the physical significance of order (q) when calculating the Information entropy?

In information theory, the entropy of a variable is the amount of information contained in the variable. One way to understand the concept of the amount of information is to tie it to how...

01 May 2020 6,703 6 View

What are the simulation tools for IoT ?

For academic purpose, free and open source simulation tool for internet of things

09 April 2020 7,137 11 View

How to identify the concentration and weight used for magnetic composite preparation ?

I have got a publication in which the authors prepared BaFe12O19/CoFe2O4. But the amount / concentration used for preparing the metal nitrate solutions were not mentioned in the publication...

15 January 2020 3,563 2 View

How to find the human chromosome DNA sequence ? Where I can get exact data ?

I already found the human chromosome DNA sequence from the ensemble browser .But the problem is in that sequence it shows some N terms are coming top , bottom and in the middle .Without N terms...

12 December 2019 7,218 4 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View