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Questions related from Vishnu Kanth
We do G-CSF fermentation using recombinant E.coli cells. After inoculating into the fermenter following a successful media hold, Dissolved oxygen % is dropping to zero within three hours. Oxygen...
12 November 2017 3,694 11 View
If a spectrophotometer is used continuously for three days without turning the lamps off in between, how could it affect the readings?
28 June 2017 743 3 View
What are the chances of impurities at the end stage chromatography? What all can lead to this? How to rectify?
18 March 2017 9,015 3 View
How to calculate lysis efficiency during microbial cell lysis by high pressure homogenizer?
18 March 2017 5,614 0 View
What happens to the cells/yield, if high pressure homogenizer passes (For microbial cell lysis and release of intracellular product) are increased more than needed?
18 March 2017 1,268 0 View
Can someone please explain Measurement, input, offset and slope of a pH transmitter. My transmitter gives slope in the range of 12-25% mA. What does that mean? How do I compare it with 92-102%...
08 July 2016 4,914 4 View
Inline pH probe and offline desktop pH probe, calibrated with same buffers show different pH of a solution at the same time. What can be the reason?
24 June 2016 3,176 4 View
Which bacteria look similar to E coli under 100X optical microscope? In a E coli fermentation culture, which contaminants look like E coli? How can we distinguish them?
18 February 2016 4,545 4 View
Does a new pH probe need polarization before use? We need to polarize a DO probe before use. What steps are to be followed to use a new pH probe?
24 October 2015 4,335 2 View
Details of IVCC needed
20 July 2014 9,285 4 View
Antifoam concentration and it´s effects on growth of E.coli.
06 April 2013 640 3 View
During cascading events of a fermentation, agitation and aeration are at a max. Thus the saturation of air with those conditions give a higher DO value compared to the saturation using basic...
28 February 2013 2,388 10 View
Water and vapor inter conversion inside the filter in between the membranes is unclear to me. Can anybody help me out?
28 February 2013 7,014 2 View
Which factor would play the major role if culture of E.coli is grown in ready made media (LB) and media made of raw materials (yeast extract and dextrose)?
27 February 2013 4,773 0 View
Fed Batch fermentation and 10L working volume.
25 February 2013 7,321 10 View
