There are 3 ways of degassing buffers: vacuum, sparging, and sonication.
In vacuum degassing, the buffer is placed in a vacuum until the dissolved gases diffuse out. This is commonly done in combination with filtration, using a vacuum filtration flask. HPLC equipment usually has a built in vacuum degassing system.
In sparging, helium is bubbled through the solution.
In sonication, the container of the solution is placed in a sonicator bath.
There are 3 ways of degassing buffers: vacuum, sparging, and sonication.
In vacuum degassing, the buffer is placed in a vacuum until the dissolved gases diffuse out. This is commonly done in combination with filtration, using a vacuum filtration flask. HPLC equipment usually has a built in vacuum degassing system.
In sparging, helium is bubbled through the solution.
In sonication, the container of the solution is placed in a sonicator bath.
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