How to calculate the CFU/ml for Mycobacterium tuberculosis growing in 7H9 broth?

I am running an experiment in which I need to calculate the CFU/ml of a Mycobacterium tuberculosis growing in 7H9 broth. Is there a reliable method to calculate cfu/ml for MTB since a culture of...

22 January 2021 10,027 2 View

How to quantify defect state density from PL spectra?

How to quantify the defect state density from PL spectra for a semiconducting thin film?

20 April 2017 7,348 5 View

Nuclear fractionation? I want see Cyclin B1 levels in cells arrested in G2/M phase. Does nuclear fractionation is required for this?

18 March 2017 337 5 View

How to perform Grouping of Pairwise comparison obtained after Tukey Test/One way ANOVA?

I am using Origin 9.1 software for one way ANOVA of my data. The data is attached, I want to compare the mean using Tukey test and represent the significant difference among the means (of control,...

14 January 2016 6,983 6 View

In vitro antioxidant assays-waste of money and time?

Dear All, Recently, I have attended two conferences in Europe (EuroFedLipid, Florence, Italy and EuroFoodChem, Madrid, Spain). During many presentations on ANTIOXIDANTS, I have observed that, many...

25 October 2015 2,016 93 View

WHy do I get High back pressure in my HPLC system after the column?

I am using Agilent 1200 HPLC-DAD system. When the detector end of the column is not connected to the detector line, the regular pressure in my HPLC is 53 bar at 1 ml/min flow rate (with column but...

26 March 2015 712 15 View

How does gravity affect the Schrodinger wave function?

How does gravity affects Schrodinger wavefunction?

01 March 2015 8,248 13 View

Can anyone provide me JCPDS XRD files for Ag and Au Nanoparticles?

25 January 2015 6,363 18 View

What is causing Dark Flow?

Dark flow is an astrophysical term describing a possible non-random component of the peculiar velocity of galaxy clusters. The actual measured velocity is the sum of the velocity predicted by...

22 January 2015 606 4 View

I have proved Heisenberg uncertainty principle incomplete in my manuscript Certainty Principle Using Complex Plane, Which institute should I approach?

09 August 2014 1,614 15 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View