17 Questions 86 Answers 0 Followers
Questions related from Mahesha M. Poojary
I am using Origin 9.1 software for one way ANOVA of my data. The data is attached, I want to compare the mean using Tukey test and represent the significant difference among the means (of control,...
15 January 2016 7,175 6 View
Dear All, Recently, I have attended two conferences in Europe (EuroFedLipid, Florence, Italy and EuroFoodChem, Madrid, Spain). During many presentations on ANTIOXIDANTS, I have observed that, many...
26 October 2015 2,159 93 View
Our system was shut down for few months. Now I am trying to tune the MS. I am new to Polaris Q. I started with Tune option and all controls-passed. Vacuum is under the required range. But, I dont...
13 August 2015 7,494 8 View
Please share your ideas on possible methods of free amino acid extraction. Normally we use dil HCl for extraction. I wish to extract from other medium. Can anyone suggest what are the other...
04 August 2015 3,731 10 View
Hi, I am running multi-level calibrartion to analyse 10 analytes in a sample. Following steps I have performed. 1. I prepared calibration standards mix at 5 different concentrations and run GC...
02 August 2015 554 8 View
Hi, I am planning to analyze amino acids using EZ: FAAST kit of phenomenex, by GC-MS and then HPLC. Can you please share your experience with this kit? Is it a good choice or not? Thanks.
02 June 2015 9,581 15 View
Which method is more suitable for analysis of free amino acids? GC or HPLC? Could you please share some GC and HPLC protocols/articles?
02 June 2015 5,055 3 View
Hi, Can anybody put their views on the analysis of nucleotides (GMP guonosine monophosphate, AMP, IMP, XMP etc.) using gas chromatography? Please share some protocols/articles. Do you think HPLC...
02 June 2015 4,723 8 View
I am using Perkin Elmer 8500 to find out MP profile of a fat. I just want to know how to find out SFI or SFC using these curves. I understood that % partial area of the curves will be used to find...
13 April 2015 481 2 View
I am using Agilent 1200 HPLC-DAD system. When the detector end of the column is not connected to the detector line, the regular pressure in my HPLC is 53 bar at 1 ml/min flow rate (with column but...
27 March 2015 894 15 View
My sample is soluble only in toluene. The UV detection wavelength is 208 nm. But UV cut off for toluene is 285 nm. Can I use toluene just to dissolve samples? I have to do 5-10 microL injection. I...
05 March 2015 4,637 7 View
I need JCPDS file for Ag and Au Nanoparticles (NPs were synthesized using biomaterials). Literature says that for Ag NPs, JCPDS file no. is JCPDS file no. 04-0783, while for Au NPs it is...
26 January 2015 6,555 18 View
I am working on an experiment using Response Surface Methodology (Design of Experiment). I use StatEase software for the same. During analysis the software shows that CUBIC model for my data is...
27 October 2014 732 18 View
I am doing an experiment, where I am optimizing a method for GC condition optimization for QUNATIFICATION of certain compounds. 1. Is it necessary to Validate the method in terms of Linearity,...
21 October 2014 2,488 15 View
Can I keep the GC column inside the GC if I am not using it for 15 days or more with carrier gas flowing and oven off? I do not want to remove the column connection from the detector and injector....
03 March 2014 501 10 View
I need a method for detection and quantification of tocopherols and sterols in oil. I do not have access to HPLC. So I need a method using GC.
28 February 2014 6,309 8 View
I am using a Varian GC 3400 Cx, having two injectors A and B. Unfortunately the system is not showing any option to turn on the Injector B in GC configuration option. Could you please tell me how...
27 February 2014 7,243 11 View
