Nuclear fractionation? I want see Cyclin B1 levels in cells arrested in G2/M phase. Does nuclear fractionation is required for this?

More Mahesha Bandara's questions See All

What are the alternative methods to measure the filler dispersion in a rubber composite other than dispergrader?

Hi everyone, I would like to seek some answers from you for my question above mentioned. If you know something let me know. Cheers

23 February 2024 2,397 2 View

I want to know how to prepare solution that use for autozero and base collection in the uv spectrophotometer?

I have dissolve my extracts using DMSO and I got absorbance that increase with the concentration. I have dough when I prepare measurements in the spectrophotometer I only use 2 sample of DMSO and...

12 February 2024 2,411 0 View

Create a Use Case Diagram that illustrates the use cases for the following Banking Application?

Customers are downloading and using the Banking App. The Bank is going to provide information that feeds into the Banking App, like transactions and account balances. The Banking App is allowing a...

26 April 2023 9,172 1 View

How we found monthly, annual or seasonal rainfall data in Hambantota area in this Pandemic situation?

2000 to 2021 rainfall data

14 September 2021 3,477 6 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,564 3 View

Are there any Statistical Quality Controlling researches based on the survey?

I want to know about uses of Statistical Quality Controlling techniques in the industries. And I hope to study this matters on survey with questionnaire.

26 December 2020 3,476 3 View

Looking for a Free, High Performance Computing Facility?

Dear All, I'm looking for freely available HPC facility to do a MD simulation for a short time period. Is there anyone, who can help by providing the access to the HPC to do the simulation.....

30 September 2019 7,566 3 View

Can you change the desorption temperature by changing the pressure?

When desorbing water from saturated silica gel, can you reduce the desorption temperature by decreasing the pressure of the gas around the adsorbent (silica gel) ?

30 May 2019 3,177 3 View

What is the best reliable primers set /protocol for SYBR Green I based quantitative detection of Dengue and nested PCR for sero-typing?

Presently I'm using Lanciotti et al., protocol for detection of Dengue using SYBR green1 PCR and I need to extend it for quantification of copy number / viral load in patient serum samples. I...

19 September 2018 8,872 1 View

ANN does not process information.It only represent information. ?

Are there any research improvements related to attention, emotions, motivation, pleasure, consciousness and personality developed using ANN.

21 August 2018 9,187 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Jaya Aseervatham

I dont thinks so. The cell lysis should be enough, but you can try with nuclear fraction for your comparison

Shail K Chaube

No, you can take a cell lysate and do the WB or fix the cells and carry out ICC to analyze cycling B1. For detail you can see our recent publications on cycling b1 using Oocytes.

Mahesha Bandara

Is that because the nuclear membrane degrades in M phase?

Ya you are right.

Thanks Jaya and Shail