Are preprint articles in "bioRxiv" (operated by Cold Spring Harbor Laboratory) citable?

The site (https://www.biorxiv.org/about-biorxiv) mentions that these articles are not peer-reviewed, edited, or typeset before being posted online. However, there are these articles in bioRxiv...

01 February 2019 8,687 1 View

How to interpret minimum free energy (MFE) values for miRNA:mRNA duplex?

What does a higher negative ddG and a positive ddG indicate with regards to binding of miRNA to mRNA? What can be used as a reasonable threshold of MFE to signify actual disruption or enhancement...

08 September 2018 9,031 0 View

What is the half life of microRNAs?

10 November 2016 5,082 1 View

Why is uracil-N-glycosylase used in qPCR?

07 August 2015 5,612 4 View

Which are the databases with experimentally validated miRNAs?

Also are there any sources to know the tissue-specific expression of miRNAs in mouse and human?

31 December 2014 9,913 3 View

How does one use PHASE 2.1 for calculating recombination rate, in Win OS?

I need to calculate the recombination rate for the biallelic SNPs in a gene of interest. However, I would like to run the PHASE programe on a Windows OS. How can we make the .inp file? Do we need...

06 July 2014 2,757 0 View

Suggestions for using Sox-2 antibody [(Y-17): SC-17320, SC] for WB ?

Hi All, I'm trying to do a western blot analysis for the detection of Sox-2 in stem cell lines, but i have failed many times over. I kindly need some help and suggestions. I'm using Sox-2...

09 October 2011 8,194 4 View

DNase treatment of LCM RNA

Hi All, After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield...

06 July 2011 9,065 8 View

Comprehensive database for epigenetic genes in human and mouse

Dear All, I am looking for a Comprehensive database for all the known epigenetic genes in human and mouse. Though I did come across some which were specific for certain types of cancers, I...

05 June 2011 9,561 0 View

To quantify cDNA or total RNA for real-time gene expression analysis?

Hi All, Im a newbie to real-time pcr. My task is to optimise a real-time gene expression assay for two GOIs expressed in mouse brain astrocytes. I had quantified cDNA after RT-PCR using Qubit...

05 June 2011 792 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View