Are preprint articles in "bioRxiv" (operated by Cold Spring Harbor Laboratory) citable?

The site (https://www.biorxiv.org/about-biorxiv) mentions that these articles are not peer-reviewed, edited, or typeset before being posted online. However, there are these articles in bioRxiv...

01 February 2019 8,687 1 View

How to interpret minimum free energy (MFE) values for miRNA:mRNA duplex?

What does a higher negative ddG and a positive ddG indicate with regards to binding of miRNA to mRNA? What can be used as a reasonable threshold of MFE to signify actual disruption or enhancement...

08 September 2018 9,031 0 View

What is the half life of microRNAs?

10 November 2016 5,082 1 View

Why is uracil-N-glycosylase used in qPCR?

07 August 2015 5,612 4 View

Can anyone help with Cotton or Polyester swabs for DNA isolation from human cheek cells?

06 July 2014 5,643 3 View

How does one use PHASE 2.1 for calculating recombination rate, in Win OS?

I need to calculate the recombination rate for the biallelic SNPs in a gene of interest. However, I would like to run the PHASE programe on a Windows OS. How can we make the .inp file? Do we need...

06 July 2014 2,757 0 View

Suggestions for using Sox-2 antibody [(Y-17): SC-17320, SC] for WB ?

Hi All, I'm trying to do a western blot analysis for the detection of Sox-2 in stem cell lines, but i have failed many times over. I kindly need some help and suggestions. I'm using Sox-2...

09 October 2011 8,194 4 View

DNase treatment of LCM RNA

Hi All, After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield...

06 July 2011 9,065 8 View

Comprehensive database for epigenetic genes in human and mouse

Dear All, I am looking for a Comprehensive database for all the known epigenetic genes in human and mouse. Though I did come across some which were specific for certain types of cancers, I...

05 June 2011 9,561 0 View

To quantify cDNA or total RNA for real-time gene expression analysis?

Hi All, Im a newbie to real-time pcr. My task is to optimise a real-time gene expression assay for two GOIs expressed in mouse brain astrocytes. I had quantified cDNA after RT-PCR using Qubit...

05 June 2011 792 11 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View