13 Questions 32 Answers 0 Followers
Questions related from Linda Koshy
The site (https://www.biorxiv.org/about-biorxiv) mentions that these articles are not peer-reviewed, edited, or typeset before being posted online. However, there are these articles in bioRxiv...
02 February 2019 8,787 1 View
What does a higher negative ddG and a positive ddG indicate with regards to binding of miRNA to mRNA? What can be used as a reasonable threshold of MFE to signify actual disruption or enhancement...
09 September 2018 9,101 0 View
Does imperfect base-pairing delay microRNA degradation in animals, as compared to plants that have a perfect base-pairing of miRNAs with target mRNAs?
11 November 2016 5,156 1 View
Likewise, Uracil-N-glycosylase cannot be used in One-Step RT-PCR. But it can be used in Two-Step RT-PCR if dUTP is not used in the RT step. Do some RT mixes use dUTP for reverse transcribing RNA?...
08 August 2015 5,760 4 View
Also are there any sources to know the tissue-specific expression of miRNAs in mouse and human?
01 January 2015 9,995 3 View
I need to calculate the recombination rate for the biallelic SNPs in a gene of interest. However, I would like to run the PHASE programe on a Windows OS. How can we make the .inp file? Do we need...
07 July 2014 2,839 0 View
Hi, could anyone suggest if cotton or polyester swabs would enable greater recovery of buccal cells and the subsequent higher quantity of DNA isolated?
07 July 2014 5,714 3 View
Hi All, I'm trying to do a western blot analysis for the detection of Sox-2 in stem cell lines, but i have failed many times over. I kindly need some help and suggestions. I'm using Sox-2...
10 October 2011 8,267 4 View
Hi All, After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield...
07 July 2011 9,146 8 View
Hi All, Im a newbie to real-time pcr. My task is to optimise a real-time gene expression assay for two GOIs expressed in mouse brain astrocytes. I had quantified cDNA after RT-PCR using Qubit...
06 June 2011 871 11 View
Dear All, I am looking for a Comprehensive database for all the known epigenetic genes in human and mouse. Though I did come across some which were specific for certain types of cancers, I...
06 June 2011 8,443 0 View
06 June 2011 9,631 0 View
Can anyone suggest suitable references that discuss or empirically prove this phenomenon, with regards to the role of microRNAs in the human genome?
01 January 1970 2,909 0 View
