IT is difficult to separate cell pellet from other sticky material
Hey, check this paper, they do RNAseq:
"2.4. RNA Preparation and RNA Sequencing
Total RNA from P. acnes (KPA171202 (type IB), and 12.1.L1 (type IA)) grown under anaerobic conditions to the exponential and stationary growth phase in BHI medium at 37 °C was prepared as described previously [43]. The cDNA libraries were constructed by Vertis Biotechnology AG, Freising, Germany, as previously described [44,45]. Sequencing was carried out with a HiSeq 2000 instrument (Illumina, San Diego, CA, USA). Detailed descriptions of procedures used for quality control, read mapping, expression analyses and data normalization have been published previously [44]. For graph visualization the Integrative Genome Browser (IGB v8.5.4) was used [46]." The Skin Bacterium Propionibacterium acnes Employs Two Variants of Hyaluronate Lyase with Distinct Properties
Seven Nazipi, Kristian Stødkilde, [...], and Holger Brüggemann
Look at the following article;
Microorganisms. 2017 Sep; 5(3): 57.
Published online 2017 Sep 12. doi: 10.3390/microorganisms5030057
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