Can anyone help me to identify this microbe with a green pigmentation?

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Phil Geis

Colony morphology isn't typical of a blue green "alga". Looks more ,ike a vibrio on TCBS.

Ivan Russkikh

Oh, but these medias are not TCBS.... And shape of the cells is bacilli.

Gram stain? What is pigmentation if incubated in the dark?

Maybe Chromobacterium?

Gram-, also green. More similar to Pseudomonas.

Maybe - tho' seems for a pretty green Pseudomonad. Any odor?

Run the usual pseudomonas tests - oxidase, etc.

Thank you very much. No, no any specific odor of Pseudomonas. Under the results with all the tests it's most similar to see the monos florists but color is very unusual and nitrogen fixation...

Catalase? Maybe try the King Agars.

Could try PIA or cetrimide but thos can be equivocal.

Catalase+. High level of producing pioverdine on King B.

16S?

So, this green bacterium has been identified as Pseudomonas brassicacearum, black is a Staphylococcus succinus.

Thanks fore the update Ivan. Can you remind - what were the media?

M9 salt glucose agar (transparent on photo) and Ashby's Sucrose Agar (with calcium carbonate - not transparent on photo).

What was the source of those isolates?