How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How will AlphaFold change the Protein Structure Prediction problem based researches in the future?

11 January 2021 9,600 4 View

Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

Performance of Reward functions?

I want to know, how we can estimate about best reward function (RF) from the list of RFs? let suppose, I have two reward functions, which one can give the best performance. 1st RF Loss = sum i=0...

25 March 2020 6,047 2 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

What is the solution to the error while running MaxQuant ?

Dear all, I use label-free QTOF technique (Bruker) in my project. I got a good result at the first time I analyzed .d folder using MaxQuant version 1.6.5. However, in the last 2 months, error...

18 August 2019 5,009 6 View

Good 260/280 number (1.9), but low individual numbers?

Hello, I performed midiprep in order to purify plasmid DNA from Ecoli DH5-alpha and obtained 72ug of the plasmid DNA. Nanodrop results showed good 260/280 ratio of 1.9, indicating a fair purity...

01 April 2018 1,790 4 View

Can someone help with a problem when I simulated this following structure in COMSOL?

Hi, I want to simulate structure from the picture in COMSOL. It is NOR logic gate based on photonic crystals. A and B are inputs, C is probe and it is always 'on', and D is output. Problem is when...

04 January 2018 2,740 4 View

How can I find temperature dependent properties of a material(for example steel)?

I want to simulate a heat treatment with Abaqus. so I need a high range of temperature date of material. thank you.

10 December 2017 5,310 4 View

What does the purpose of land use/cover change by remote sensing?

land use change detect by using landsat images

28 October 2017 5,588 8 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View