When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not labelled antibody; labelled antibody and not labelled antigen. What is the formula you need to use, what to do with the mix of antibody/antigen/labelled/

unlabelled and why would you need to do it?

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