Is this right way to get Modulation Transfer Function(MTF)?

Hi I'm trying to make a python script to get MTF curve for the input image Here is my algorithm to get MTF. 1. get Image 2. select ROI(High contrast region) 3. extract a center line out of image...

15 August 2020 6,646 3 View

How can I use with ECMWF wind and pressure in MIKE 21 HD modelling and having sinusoidal curve of surface elevation?

I have ECMWF ERA-5 wind and pressure data in NetCDF file, and i was able to convert the data to dsf2 file for use in MIKE 21 HD model. The problem that the results of the simulation (surface...

19 November 2019 3,850 3 View

What is the solution to the error while running MaxQuant ?

Dear all, I use label-free QTOF technique (Bruker) in my project. I got a good result at the first time I analyzed .d folder using MaxQuant version 1.6.5. However, in the last 2 months, error...

18 August 2019 5,009 6 View

Can anyone help me to find a rapid sensitive assay for quantitative methylation analysis ?

Hello all, in my research I need to do quantitative methylation analysis on 3 candidate genes and have to do it in only 4 months, can you please help to find a fast but sensitive method for that?...

19 April 2019 7,728 3 View

Good 260/280 number (1.9), but low individual numbers?

Hello, I performed midiprep in order to purify plasmid DNA from Ecoli DH5-alpha and obtained 72ug of the plasmid DNA. Nanodrop results showed good 260/280 ratio of 1.9, indicating a fair purity...

01 April 2018 1,790 4 View

How can I find temperature dependent properties of a material(for example steel)?

I want to simulate a heat treatment with Abaqus. so I need a high range of temperature date of material. thank you.

10 December 2017 5,310 4 View

Why do I get a wrong length of PCR result but after sequencing it is actually a right target sequence?

Can somebody help me? I am doing a PCR of worm tissue. I used KAPA Taq Extra HotStart ReadyMix. I should be getting a PCR result of 158bp but I always get a 200 bp PCR result and after I sequence...

15 June 2016 7,313 6 View

Can anybody recommend companies that generate custome gene knockout cell lines using CRISPR?

I need to knock out a gene in a human cancer cell line using CRISPR. This knockout cell line is not available commercially so I want to find a company to do the job. There are some companies that...

25 August 2015 5,887 3 View

What kinds of secondary antibodies are recommended for western blotting using odyssey infrared imager?

Now many companies are providing IRD800 or IRD700 or alexa fluor 680 dye labelled secondary antibodies. What is the best? Could you recommend a company for western blotting using odyssey infrared...

01 February 2014 6,000 3 View

Can you conduct a stable transfection using lipofectamine? Also how do you screen the cells afterwards?

I am facing some problems understanding the mechanincs of stable transfection.

05 May 2012 5,793 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View