I am working on metabolic engineering. I am trying to express some genes from plants to my host bacteria. I have a mutant with one heterologous gene. This mutant showed different phenotype from wild type in liquid culture while producing some compounds in stationary phase in the presence of sucrose ( sucrose added in log phase). I want to analysis which type of compound/s is/are produced in liquid culture.

Can anyone help me to develop/design methods to analysis the unknown compounds by using GC-MS or HPLC? Or anyone have any idea about other most suitable scientific techniques for this type of analysis?

Please give me your valuable suggestions.

Thanks all in advance.

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