I need measuring calculation of all this ingredients
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
ΔG = 12.77 ΔH = -37,92 ΔS = -162,40
07 May 2024 4,942 3 View
Hello, everyone I've recentely isolated bacteria from pigeon pea (Cajanus cajan) nodules on YMA medium and then transferred some colonies to YMB (Yeast Mannitol Broth) medium. This is the first...
01 May 2024 3,296 4 View
I ran a qualitative DNA agarose gel 1% to see if my DNA extraction had been done correctly. I am not sure if the DNA shown on the gel below is too concentrated or it has some degradation because...
15 April 2024 3,942 0 View
I'm trying to run the Structure Harvester for interpretation based on Evanno method and the website don't work. Someone have any alternatives? Thank you
12 April 2024 2,757 1 View
Exploring strategies to empower leaders: How can organizations cultivate a culture that encourages effective problem-solving despite leadership apprehension?
28 March 2024 457 8 View
Exploring the evolution of research leadership amidst global challenges: How can leaders foster collaboration and ethical decision-making?
28 March 2024 7,059 0 View
Analyzing the Role of AI Adoption in Healthcare and Its Implications for Patient Outcomes
28 March 2024 3,267 2 View
I want to see adipocytes from this material in slides with oil red. I can decalcify and place in OCT by making cuts on silanized slides? How thick is the cut? Can my cryostat cut decalcified...
07 March 2024 1,743 0 View
Hello everyone! I work in a project where I need to label lipids at the membrane, more specifically gangliosides, and now I would like to check the localization of these same lipids in relation...
13 February 2024 3,229 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey
05 August 2024 5,332 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
