What is the effect on the conductivity of the thin film during the process of thermal evaporation ?

Why thermally evaporated (VCM 600 V1 configuration of the thermal evaporator) CdTe film found n-type not p-type conductivity whereas all CdTe films deposited by varying the source to substrate...

23 November 2020 7,427 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Monolitic construction of brick masonary and concrete simultaniously have any effect ?

Concrete column casting after masonary completed monolithic.

17 August 2020 7,172 3 View

Rentraint Corner of building if need extra stitching diagonal reinforcement ?

If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.

12 August 2020 1,707 2 View

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26 July 2020 1,511 0 View

How to calculate Time Average data (WSS, Velocity, Pressure) in ANSYS Fluent ?

I am doing a blood flow research for which Time average data of Velocity and Wall shear stress is important. The study is transient and my flow time is 0.5 sec. I save data with time step size of...

07 July 2020 4,323 2 View

What is the best management strategy for Diamond Back Moth (DBM) in cole crops?

What should be the best managemnt option for suppressing the DBM problem that farmer can take into consideration?

11 May 2020 4,280 1 View

Is it a good idea to retract a submitted article as the journal is taking too much time to make a decision?

I had submitted a research article related to the field of mechanical engineering (CFD) on the last December, 2019 in a Scopus Indexed journal. It has been around 6 months but the journal...

11 May 2020 9,286 8 View

Where can I get details of SARS-CoV-2 infected patients?

I have been working on peptide vaccine for SARS-CoV-2 or COVID-19 and also recently published some analysis on mutational changes in this virus. It would have been really helpful if I could get...

05 May 2020 2,749 9 View

How does the chemical etching (using HF) affect the temperature sensitivity of a fiber Bragg grating (FBG) inscribed in silica fiber?

Due to chemical etching, the cladding diameter of the silica fibre decreases with time. How does this reduced cladding diameter affect the temperature sensitivity of an FBG, which is inscribed on...

17 April 2020 4,771 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View