I have taken fluorescence images of the control and treated sample(Immunofluorescence, tissue sample) at the same settings. So I need to measure the change in fluorescence intensity of the treated cells as compared to the cells in control
Samir Ranjan Panda
Hi Jhilik Dey
Here's how to measure mean fluorescence intensity in a few simple steps, assuming you're using Fiji (ImageJ) and analyzing a confocal microscopy image:
1. Pick your image area:
- Open your image in Fiji.
- Decide on the specific area you want to measure. This could be a whole cell, a specific part of a cell, or a defined region.
2. Draw your selection:
- Use the selection tools in Fiji to draw a line or shape around the area you want to measure.Freehand drawing tool lets you draw a custom shape around your area. Existing selections can be used if you already have a mask or outline highlighting your region. Line selection tool is useful if you want to measure intensity along a specific line.
3. Measure the intensity:
- Once you have your area selected, go to the Analyze menu and choose Measure.
- A window will pop up with various measurements. The Mean value represents the average fluorescence intensity within your chosen area. This is your mean fluorescence intensity.
Hope it helps,
Thanks,
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