I want to know the ways by which i can increase expression and transient transfection efficiency of CHO cells.
Please let me know any efficient additives, Temperature condition, Transfection Kits that has worked as per experience.
Lipid-mediated transfection of CHO cells is generally very efficient (around 80% for siRNA with this kit: https://altogen.com/product/cho-transfection-reagent-chinese-hamster-ovary-cells/). Be sure to consider the effects of the plasmid you are transfecting, as that can affect cell viability afterwards. Transient transfection is far easier to achieve than stable transfection, so you should be able to get high efficiencies.
Hi Santosh,
CHO cells are very easy-to-transfect and you may obtain >80% efficiency with any chemical reagent available on the market. Of course, you can always adjust parameters to further increase your transient transfection rates, e.g. cell density, amounts of complex, ratio reagent:DNA, time before read-out.
For instance, you can have a look to the manual of our Viromer RED which achieves around 80% efficiency for pDNA in CHO cells. Another way to increase the final protein level is to work with mRNA. Here, the Viromer RED shows almost 100% of positive cells (using a GFP encoding mRNA).
https://viromer-transfection.com/mrna-transfection
Good luck! And do not hesitate if you need more info about our technology.
Bests, Sandra
Hi Sandra,
I am obliged for your timely response and valuable input. Will definitely try this as data is very much convincing.
Thanks for such a wonderful product.
Regards,
Santosh kumar
Hi Santosh,
I am learning more about this area every day, but have read some interesting papers recently in which they used HDAC inhibition to increase CHO protein expression. Look into it, as there are some relatively cheap ways of doing this. Best of luck!
Thomas
Lipid-mediated transfection of CHO cells is generally very efficient (around 80% for siRNA with this kit: https://altogen.com/product/cho-transfection-reagent-chinese-hamster-ovary-cells/). Be sure to consider the effects of the plasmid you are transfecting, as that can affect cell viability afterwards. Transient transfection is far easier to achieve than stable transfection, so you should be able to get high efficiencies.
Hi Matt,
Thanks for your response.
Please help me understand few more associated concepts herein.
I have dropped mail to inbox regarding.
Regards,
Santosh
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