Does ozone layer present in uppermost layer of earth atmosphere?oxygen is below it ? However ozone molecular weight greater than oxygen? Am i Right?

Biotransformation

10 November 2017 9,220 3 View

What is possibility of detection by ELISA?

Hi, In one of bizarre finding, I got no binding in ELISA for one set of mAb expressed transiently, however positive and negative including Test2 sample gave expected results. Further when...

10 November 2017 7,117 2 View

Can anyone help me increasing transient expression (Lipid mediated transfection) of protein in CHO cells?

I want to know the ways by which i can increase expression and transient transfection efficiency of CHO cells. Please let me know any efficient additives, Temperature condition, Transfection Kits...

10 November 2017 6,080 6 View

Detection of Agonist, Partial Agonist and Antagonist antibody type?

Hi All, I am dealing with the problem of having mixed nature of monoclonal antibody in the system that is in terms of its activity of showing efficacy. I would like to know if someone could help...

08 September 2016 9,772 3 View

Is it possible that if cell bank were made from suppose X medium had 98% Viability however on revival on same media not supporting growth?

My cloned cells are not supporting growth using the same media. It was kept in cryopreserved state in cell banks. Can any one help me to get an idea like how its possible? pH is maintained thus...

06 July 2016 1,745 2 View

What is the role of nitrogen source for growth of bacteria in media?

I am working on Bioremediation.

01 February 2016 7,007 2 View

What are the different methods to preserve cells for long time & why used different method to preserve cell ?

I am working on Bioremediation

31 December 2015 8,076 1 View

How to make standard graph of any reagent or molecule or compound ?

I am working on Bioremediation.

31 December 2015 3,366 2 View

If microorganism present in media and then autoclave that media. Is there any change in the media composition due to denatured protein of bacteria?

I am working on bioremediation.

31 December 2015 3,967 3 View

What is the principle of estimation of nucleic acid?

I am working on Bioremediation.

31 December 2015 715 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View