Thomas J Esparza

3 Questions 19 Answers 0 Followers

Questions related from Thomas J Esparza

Sequencing phage display clones yields M13 sequence and not phagemid?

I am performing phage display and I occasionally find that some of my ELISA positive clones when isolated and sequenced give me phage sequence and not the phagemid. Any experience with this? Any...

07 July 2019 6,789 4 View

Can anyone recommend a good secretion signal sequence for protein expression in CHO or HEK cells?

Looking to express and purify single-domain antibodies in a mammalian system and would like to have them secreted into the media.

11 November 2017 4,112 1 View

Best Streptavidin-HRP reagent?

I have recently encountered a problem with acquiring Streptavidin-HRP 20 from Fitzgerald. They have been our standard detection reagent for ELISA assays for a number of years, however I want to...

10 October 2013 2,198 3 View