Santosh Kumar

12 Questions 19 Answers 0 Followers

Questions related from Santosh Kumar

What is possibility of detection by ELISA?

Hi, In one of bizarre finding, I got no binding in ELISA for one set of mAb expressed transiently, however positive and negative including Test2 sample gave expected results. Further when...

11 November 2017 7,187 2 View

Can anyone help me increasing transient expression (Lipid mediated transfection) of protein in CHO cells?

I want to know the ways by which i can increase expression and transient transfection efficiency of CHO cells. Please let me know any efficient additives, Temperature condition, Transfection Kits...

11 November 2017 6,151 6 View

Does ozone layer present in uppermost layer of earth atmosphere?oxygen is below it ? However ozone molecular weight greater than oxygen? Am i Right?

Biotransformation

11 November 2017 9,350 3 View

Detection of Agonist, Partial Agonist and Antagonist antibody type?

Hi All, I am dealing with the problem of having mixed nature of monoclonal antibody in the system that is in terms of its activity of showing efficacy. I would like to know if someone could help...

09 September 2016 9,849 3 View

Best media for CHO cell lines?

Can anyone help me to get which media i should use to get maximum biomass for CHO cell lines?

07 July 2016 4,119 1 View

Is it possible that if cell bank were made from suppose X medium had 98% Viability however on revival on same media not supporting growth?

My cloned cells are not supporting growth using the same media. It was kept in cryopreserved state in cell banks. Can any one help me to get an idea like how its possible? pH is maintained thus...

07 July 2016 1,812 2 View

What is the role of nitrogen source for growth of bacteria in media?

I am working on Bioremediation.

02 February 2016 7,078 2 View

What are the different methods to preserve cells for long time & why used different method to preserve cell ?

I am working on Bioremediation

01 January 2016 8,146 1 View

How to make standard graph of any reagent or molecule or compound ?

I am working on Bioremediation.

01 January 2016 3,438 2 View

What is the principle of estimation of nucleic acid?

I am working on Bioremediation.

01 January 2016 806 5 View

If microorganism present in media and then autoclave that media. Is there any change in the media composition due to denatured protein of bacteria?

I am working on bioremediation.

01 January 2016 4,059 3 View

How does the detector of HPLC give peak of the sample run in column ?

I am working on bioremediation.How does the detector of HPLC give peak of the sample run in column ?

12 December 2015 1,631 3 View