Hi all,
First time using a transwell to seed my pericyte cells. I seeded 40,000 cells/well in a 6 well plate. I put media only in the inner chamber, is that correct or do I also have to place media in the well of the culture plate too? I am having trouble visualizing my cells while they are growing on the transwell. Are the round circles my cells? How would I determine confluency, same as in a culture flask? This is day 3 growing on the transwell, I thought I would see more confluency by now.
TYIA
Can you explain the purpose of the culture? If you are using it for cell-to-cell interaction, you must add medium in both the inner and outer chambers.
Muhammad Aquib Hi there, thank you for asking. I am trying to take TEER values (ultimately the plan is to culture astrocytes, pericytes and endothelial cells to mimic the BBB). This is the first layer I am culturing on transwells.
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