Ashley Amis

7 Questions 2 Answers 0 Followers

Questions related from Ashley Amis

How to probe for E-Cadherin in Western Blot?

Hi All, I have tried many times to probe for E-Cadherin in Western blot but I am unsuccessful. I am using sheep tissue (mainly aorta right now) and I use a RIPA Extraction buffer. Does anyone have...

05 August 2025 4,766 3 View

URGENT! How do I make 10mM PBS?

HI all I am trying to follow this recipe, but I do not have Na2HPO4-7H20, can I substitute this reagent with something else and how would that change my ratio? Recipe for Buffer 1: 10 mM PBS, pH...

29 July 2025 9,969 0 View

Can anyone help me identify my pericyte cells in this transwell?

Hi all, First time using a transwell to seed my pericyte cells. I seeded 40,000 cells/well in a 6 well plate. I put media only in the inner chamber, is that correct or do I also have to place...

19 June 2025 294 2 View

Transfer for alpha-actin 42kda protein?

HI all, What is the ideal conditions for protein transfer for a 42kda protein? I use the Bio-Rad mini Blot transfer system with Bjerrum Transfer buffer. I usually run transfers at 0.2A for 90...

18 December 2024 4,661 1 View

How do I add an ascorbic acid solution to my cell culture media that I can ensure is sterile ?

Hi all, I am working on creating my complete media for VSMC. I have to reconstitute 50mg of ascorbic acid in 10ml H2O (or base media) and then add 2.5ml into my final volume of 250ml complete...

01 November 2024 2,592 1 View

Does this look like Cell contamination?

Hi all, I am having trouble deciding if this is cell contamination or something else. I keep seeing this fiber like bodies in my media (not on the same level as my adherent cells). There is no...

30 October 2024 8,961 1 View

Does this look like Cell contamination?

HI all, I have started cell culture for VSMC and I saw this in my culture the other day. It does not move when gently jiggling the plate and I have seen it even after rinsing with PBS and...

25 October 2024 4,495 2 View