I would like to genotype the pellet of debris after homogenization of brain tissue with Tris-Hcl+ 1% Tritonx100 lysis buffer and centrifugation at 13000g, what will be the best protocol?
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
I am new to machine learning, I working on regression neural network to prediction the outcomes of my experiments. I created the neural network with a hidden layer to predict my outcomes, now i...
31 October 2023 4,262 2 View
Surface textures is the intentional introduction of well defined features like micro dimples This helps to generate hydrodynamic lift between the parallel surface when they are in relative motion.
22 August 2023 4,265 3 View
I am doing numerical studies of selective laser melting of AlSi10Mg, I want to incorporate the temperature dependent density, dynamic viscosity, specific heat, and thermal conductivity. I need the...
21 July 2021 9,812 4 View
I understand immunostaining for G3BP can be done but is there a way to examine stress granule formation with live imaging?
19 April 2021 6,803 0 View
Aluminium sheet of thickness 1mm is deformed with various process to compare the dislocation density.
14 November 2018 5,846 7 View
suggest me some journals to learn about the technique to do and analysis.
21 September 2018 2,303 3 View
We are collected some deer ked fly. We want to go for molecular characterization of these flies. Kindly help us to extract DNA from these samples.
25 April 2018 8,125 3 View
I´m making my master final work about teaching geometry and this is one of the model I want to explore.
14 December 2015 988 3 View
For those who have conducted Co-IP before, I would like to know the amount of protein required for Co-IP experiment. We will use lysate from cells.
29 March 2015 7,348 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View