Do we have to check the efficiency of sonication by doing a gel run everytime before proceeding for ChIP?

Sreelakshmi S Kumar @Sreelakshmi-S-Kumar

07 May 2024 0 7K Report

I am trying to optimize ChIP for my cell lines. I am doing with WT cells. I keep checking sonication efficiency on an agarose gel. So if I take a new sample of the same cell line, do I have to do gel run again before going forward with ChIP

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