We have performed SDS PAGE for separating the extracellular protein of staphylococcus aureus resistant strain MRSA... we have followed the aluminium sulphate precipitation protocol for salting out the bacterial protein.. and run the protien sample on 12.5% resolving gel...
after running for 7,8 hours on 110 volts, I got these unexplainable results... what happened at the bottom of the gel???? what could be the possible reasons???
Hello Nauman Ghumman,
It looks like that there is problem with separating gel conc. I think its not 12.5 %. From molecular marker only two bands are separated because of higher mol. wt. rest of the proteins wipe out and collected in the base of the gel.... Look again at your recipe of separating gel..
hi, i think this could be two things different
1-maybe you let too much time at 100°C with the 4 or 6X
or 2- try 15% in the concentrator.
Also, maybe you could try less protein concentration and use a 10 well comb.
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