Checking stability of a sequence by in vitro transcription/translation??

I have cloned different type of sequence(non canonical) after t7 promoter followed by a reporter gene.i am using in-vitro/transcription translation for quantifying reporter. can someone provide me...

04 May 2018 666 3 View

How to make graph for chip-qpcr data?

06 July 2017 442 2 View

How to draw DNA sequences with lines for Paper manuscript?

Generally using powerpoint. I draw lines to make DNA SEQUENCES.I work on dna a lot and most of the times, I have to make DNA sequence figures like the example file I attached but I dont know how...

11 December 2016 4,014 7 View

How to know that a particular dna sequence is preferentially fold into hairpin or quadruplex invivo/invitro?

08 September 2016 2,935 9 View

Markers for antiproliferative, adhesion, migration, invasion, apoptosis for mda mb 231?

08 September 2016 8,338 6 View

What DELTA NEGATIVE G value OF FOLDED SINGLE STRANDED DNA OLIGO Indicates?

I am using rfold software to evaluate the stability of single stranded oligo . the software gives different delta g negative value. what it indicates.

07 August 2016 5,395 4 View

Cell morphology/health when treated with chemical for doing rt pcr?

05 June 2016 2,789 7 View

Smear bands in 15 percent urea denaturing page?

05 June 2016 2,845 9 View

Is it possible to do EMSA without protein purification?

Hi, i want to see the binding of a protein to different conformation of ss DNA. Is it possible to do EMSA without purification of my protein. I have my interested protein in both mammalian and...

04 May 2016 9,507 8 View

How to distinguish by any assay between antiproliferative and cytotoxiicty of cell line( mda-mb231 )caused by chemical?

04 May 2016 9,066 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View