Checking stability of a sequence by in vitro transcription/translation??

I have cloned different type of sequence(non canonical) after t7 promoter followed by a reporter gene.i am using in-vitro/transcription translation for quantifying reporter. can someone provide me...

04 May 2018 666 3 View

How to draw DNA sequences with lines for Paper manuscript?

Generally using powerpoint. I draw lines to make DNA SEQUENCES.I work on dna a lot and most of the times, I have to make DNA sequence figures like the example file I attached but I dont know how...

11 December 2016 4,014 7 View

How to know that a particular dna sequence is preferentially fold into hairpin or quadruplex invivo/invitro?

08 September 2016 2,935 9 View

Markers for antiproliferative, adhesion, migration, invasion, apoptosis for mda mb 231?

08 September 2016 8,338 6 View

What DELTA NEGATIVE G value OF FOLDED SINGLE STRANDED DNA OLIGO Indicates?

I am using rfold software to evaluate the stability of single stranded oligo . the software gives different delta g negative value. what it indicates.

07 August 2016 5,395 4 View

Cell morphology/health when treated with chemical for doing rt pcr?

05 June 2016 2,789 7 View

Smear bands in 15 percent urea denaturing page?

05 June 2016 2,845 9 View

Can anyone guide me about the cell surface marker for ectoderm and endoderm cells?

hi, in my research, i am expecting the presence of all three germ layer cells in a petridish.is there any cell surface markers which i can used for FACS analysis so that i can determine the...

04 May 2016 3,682 5 View

Is it possible to do EMSA without protein purification?

Hi, i want to see the binding of a protein to different conformation of ss DNA. Is it possible to do EMSA without purification of my protein. I have my interested protein in both mammalian and...

04 May 2016 9,507 8 View

How to distinguish by any assay between antiproliferative and cytotoxiicty of cell line( mda-mb231 )caused by chemical?

04 May 2016 9,066 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View