Varun Bansal

11 Questions 22 Answers 0 Followers

Questions related from Varun Bansal

Checking stability of a sequence by in vitro transcription/translation??

I have cloned different type of sequence(non canonical) after t7 promoter followed by a reporter gene.i am using in-vitro/transcription translation for quantifying reporter. can someone provide me...

05 May 2018 742 3 View

How to make graph for chip-qpcr data?

I have sent my samples to some US company that does chip-qPCR. They have sent me the data but did not make the graphs. Can anyone help me on this? I have never worked on chip assay so I have no...

07 July 2017 479 2 View

How to draw DNA sequences with lines for Paper manuscript?

Generally using powerpoint. I draw lines to make DNA SEQUENCES.I work on dna a lot and most of the times, I have to make DNA sequence figures like the example file I attached but I dont know how...

12 December 2016 4,066 7 View

Markers for antiproliferative, adhesion, migration, invasion, apoptosis for mda mb 231?

I want to study the effect of a chemical on breast cancer cell lines .For example MDA-MB 231. I found one chemical , When I use that chemical I can see less proliferation of cells with...

09 September 2016 8,388 6 View

How to know that a particular dna sequence is preferentially fold into hairpin or quadruplex invivo/invitro?

I am working on a dna sequence which can fold into hairpin or quadruplex.I am confused about how to proceed this problem and invitro/invivo techniques used to establish the role of that sequence...

09 September 2016 2,974 9 View

What DELTA NEGATIVE G value OF FOLDED SINGLE STRANDED DNA OLIGO Indicates?

I am using rfold software to evaluate the stability of single stranded oligo . the software gives different delta g negative value. what it indicates.

08 August 2016 5,444 4 View

Smear bands in 15 percent urea denaturing page?

i am running a dna sequencing reaction in a 40 cm long gel but i am not able to get crisp bands.bands seems diffuse. running condition 1500 V / 1x TBE buffer. oligo length 24 base 5 end FAM...

06 June 2016 2,919 9 View

Cell morphology/health when treated with chemical for doing rt pcr?

I am trying to know the expression of a particular gene when treated with a chemical.For that i am doing rt pcr of treated and no treatment wells of cell line.My question is that if I am observing...

06 June 2016 2,861 7 View

How to distinguish by any assay between antiproliferative and cytotoxiicty of cell line( mda-mb231 )caused by chemical?

is there any possibility that cell culture media color can distinguish between cytotoxicity of cell and antiproliferative property of cell .is it true that media color dont change to yellow when...

05 May 2016 9,105 9 View

Is it possible to do EMSA without protein purification?

Hi, i want to see the binding of a protein to different conformation of ss DNA. Is it possible to do EMSA without purification of my protein. I have my interested protein in both mammalian and...

05 May 2016 9,558 8 View

Can anyone guide me about the cell surface marker for ectoderm and endoderm cells?

hi, in my research, i am expecting the presence of all three germ layer cells in a petridish.is there any cell surface markers which i can used for FACS analysis so that i can determine the...

05 May 2016 3,733 5 View