Checking stability of a sequence by in vitro transcription/translation??

I have cloned different type of sequence(non canonical) after t7 promoter followed by a reporter gene.i am using in-vitro/transcription translation for quantifying reporter. can someone provide me...

04 May 2018 666 3 View

How to make graph for chip-qpcr data?

06 July 2017 442 2 View

How to draw DNA sequences with lines for Paper manuscript?

Generally using powerpoint. I draw lines to make DNA SEQUENCES.I work on dna a lot and most of the times, I have to make DNA sequence figures like the example file I attached but I dont know how...

11 December 2016 4,014 7 View

How to know that a particular dna sequence is preferentially fold into hairpin or quadruplex invivo/invitro?

08 September 2016 2,935 9 View

Markers for antiproliferative, adhesion, migration, invasion, apoptosis for mda mb 231?

08 September 2016 8,338 6 View

Cell morphology/health when treated with chemical for doing rt pcr?

05 June 2016 2,789 7 View

Smear bands in 15 percent urea denaturing page?

05 June 2016 2,845 9 View

Can anyone guide me about the cell surface marker for ectoderm and endoderm cells?

hi, in my research, i am expecting the presence of all three germ layer cells in a petridish.is there any cell surface markers which i can used for FACS analysis so that i can determine the...

04 May 2016 3,682 5 View

Is it possible to do EMSA without protein purification?

Hi, i want to see the binding of a protein to different conformation of ss DNA. Is it possible to do EMSA without purification of my protein. I have my interested protein in both mammalian and...

04 May 2016 9,507 8 View

How to distinguish by any assay between antiproliferative and cytotoxiicty of cell line( mda-mb231 )caused by chemical?

04 May 2016 9,066 9 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View