Can anyone figure out how a molecule may activate p53 (upregulation, p53, PUMA, p21) and at the same time promote its ubiquitination?

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Claudio Brancolini

The kind of assay that are you using should be described with more details.

Dominick G.A Burton

....off the top of my head I would say check UBC13

Dominique Soroka

Human 20S Proteasome Inhibition? If chymotrypsin-like proteasome activity is inhibited, there should be an abundance of p53, also in its ubiquinated form. The two are commonly linked in cancer therapeutic studies.