Dominick G.A Burton

3 Questions 15 Answers 0 Followers

Questions related from Dominick G.A Burton

What is the mechanism by which phorbol 12-myristate 13-acetate (PMA) induces THP-1 differentiation into macrophages?

I have just started to work with THP-1 monocytes, but cannot seem to find information on the mechanism of PMA on THP-1 for differentiating them into macrophages. Does anyone know what the...

28 June 2015 9,421 6 View

Do you wait more than seven days after Dox treatment before monitoring senescence mediated calcification?

Following DNA damage and senescence induction it can take up to 7 days (Coppe et al, 2008) for the SASP to fully develop. Since it has been suggested (Burton et al 2010) and evidence provided...

23 December 2013 5,779 1 View

Can cellular senescence occur in the absence of DNA damage (but still retain a SASP) in physiological conditions?

The senescence-associated secretory phenotype (SASP) is normally a response to DNA damage, but can the SASP occur independent of DNA damage? If not, are cells that have no SASP (or DNA damage)...

09 December 2013 1,368 2 View