Why Graph Neural Networks aren't hierarchical?

Hello, I read about Graph Neural Networks, and understood that it is very similar to CNN, only that is on graphs and without pooling for downsizing. Each iteration the "convolution" is of...

12 July 2020 4,330 6 View

How can droplet particle type be enabled in ANSYS Fluent?

Hello, I'm trying to enable droplet particle type in Fluent. Energy eqn.s are turned on. However only inert and massless types are available. So, if there is anyone who faced this kind of problem...

22 May 2020 6,387 1 View

Does expired PaxGene tubes have effect on the cfDNA yield or quality?

I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...

09 March 2020 3,967 1 View

Which concentration measurement is reliable for cfDNA? Nanodrop or Qubit?

I am trying to isolate cfDNA from plasma samples (2-3 ml) for NGS. I get around 15 ng/ul concentrations of cfDNA from nanodrop but 10 times lower conc (1.4 ng/ul) from qubit dsDNA HS assay. Why...

22 July 2018 7,155 3 View

TRAP staining protocol TROUBLEshooting?

Hi everyone, I am trying to deal with an unreasonable problem with TRAP staining of differentiated RAW264.7 cells (RANKL induced). I was using a protocol for TRAP staining, and it worked perfectly...

12 March 2017 8,140 3 View

PFGE - DNAse contamination?

26 October 2016 9,269 3 View

Converging problem in transient heat analysis due to amplitude. Anybody has idea?

15 January 2016 3,765 2 View

What is the typical concentration of total proteins isolated from cell lines, say L929?

12 September 2015 476 2 View

Is there any publicly available benchmark dataset to check software code plagiarism?

A plagiarized code may be either an exact copy of the original code, or a variant, applying various textual transformations such as; - Changing the order of operands/operators in expressions,-...

02 February 2015 9,851 7 View

Can anyone tell me the ingredients of liver cancer stem cell media?

26 October 2014 9,662 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View