Deniz Kom

6 Questions 15 Answers 0 Followers

Questions related from Deniz Kom

Can RNA isolation from Covid tissues be performed at lab bench?

Hi everyone, I have Covid infected tissues and have to isolate RNA from them. But I dont have an access to BSL3 or 4 laboratory in my institute. My colleagues are prepared and fixed tissues in...

01 July 2021 6,882 5 View

Crispr gRNA primer design rotation?

Hi everybody, I am trying to create vectors with multiple gRNAs for my knocking out study. I decided to use 4 different gRNA in groups of two. But the challenging part is some of my gRNAs are in...

04 May 2020 8,812 0 View

HPLC analysis, different retention time?

Hi friends, I have a problem with HPLC analysis. I am using C18 column for isoquinoline extract analysis. Retention time of my standard sample is around 4.542, which is 4.595 at plant sample. But...

09 July 2019 4,747 5 View

How to design gRNA for non-model organisms?

Hi friends, I am trying to knock-out a goi but whenever I try to design cas9 gRNA it is turning to a disappointing event. I have full sequence of my goi and KEGG map data for metabolism of enzyme...

26 October 2017 524 2 View

How do I measure RNA purity with a nanodrop for cDNA synthesis?

Hi all, For an expression study I am trying to optimize RNA isolation and cDNA sythesis protokols. Until now I have tried Thermo Plant RNA Isolation Kit, Trizol method and CTAB method (Jaakola ét...

29 May 2015 859 4 View

Can anyone explain why signal was transmitted at Tyrosine P on PDGF receptor, then P was removed and signal transmission has to stop?

How this area is protected from kinases? I am trying to find an answer for that question. I need to understand that mechanism but so far could not manage to do it. Can anyone working with this...

01 June 2014 3,898 5 View