A monolithic HPLC column is a special type of several kinds of columns are used in HPLC with porous channels rather than beads.
Monolithic columns possess a different structure from traditional columns. Their construction is more akin to a rod with lots of random channeling and outcroppings.
Monoliths, are porous rod structures characterized by mesopores and macropores. These pores provide monoliths with high permeability, a large number of channels, and a high surface area available for reactivity. The backbone of a monolithic column is composed of either an organic or inorganic substrate, and can easily be chemically altered for specific applications. Their unique structure gives them several physico-mechanical properties that enable them to perform competitively against traditionally packed columns. Finally, Monolithic columns are more physically stable than their particulate counterparts.
A monolithic HPLC column is a special type of several kinds of columns are used in HPLC with porous channels rather than beads.
Monolithic columns possess a different structure from traditional columns. Their construction is more akin to a rod with lots of random channeling and outcroppings.
Monoliths, are porous rod structures characterized by mesopores and macropores. These pores provide monoliths with high permeability, a large number of channels, and a high surface area available for reactivity. The backbone of a monolithic column is composed of either an organic or inorganic substrate, and can easily be chemically altered for specific applications. Their unique structure gives them several physico-mechanical properties that enable them to perform competitively against traditionally packed columns. Finally, Monolithic columns are more physically stable than their particulate counterparts.
Furthermore, the high permeability of monoliths means they have a much lower back pressure than particle packed columns and can be operated at a much faster mobile phase flow rate.
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