Comparing between methanol and acetonitrile to separate sugars by LC/MS we got a better signal using acetonitrile.
NH2 column
methanol:water
acetonitrile:water
Acetonitrile will give better signal due to its organic radicals.
I use methanol: water
acetonitrile :water
water:acn on a NH2 column is a normal phase chromatography. That being said, MeOH is stronger sovent than ACN in NP chromatography. Water is the strongest, so if you want larger k values you should reduce the water % (opposite to RP chromatography).
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see above
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See above
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