I found conflicting results about use of kevetrin in tumors without wild-type p53. What if tumors carry a partial deficiency allele, such as p.S106R and a deletion of the second allele?
como se ve afectado un docente de educación superior por la falta de conocimientos digitales, sus emociones
20 May 2024 1,149 0 View
What are the current limitations regarding the antimicrobial susceptibility methods, standard procedures implementation, and workplace communication between the clinicians and the microbiology...
06 February 2024 6,318 4 View
Hello, I am performing DNA extraction from mosquitoes with an in-house protocol. During the genomic DNA electrophoresis gel test, I noticed two distinct bands. I'm curious if anyone knows the...
04 October 2023 7,739 0 View
I have a situation which quite do not understand. We are trying to get a qPCR to work. We extracted RNA and synthersized cDNA from have 5 Wts samples, 3 iRNA samples, and 1 genomic WT control. We...
24 August 2023 2,050 2 View
I need a schematic drawing about rabbit spermatogenesis. No pictures. Thanks!
07 August 2023 7,286 0 View
Hello, I'm working with highly charged supramolecular systems and there is a discrepancy of one unit between my experimental and calculated data. I'm using ChemCalc to calculate the mass spectrum...
07 June 2023 7,815 0 View
Hi, in my lab we have an old lightcycler 2.0 qc system that we want to use, unfortunately we do not have the CD, does any one know were can I download the software for this machine?. thanks to...
04 June 2023 8,087 3 View
I need to conduct a 10-page research paper on the topic of human resources, specifically I need to analyze a business case and then conduct interviews. Any idea?
31 March 2023 7,367 1 View
I was using a 3 magnetron equipment, but now I am using an equipment that has both e-beam and sputtering in the same chamber. E-beam works perfectly but I am having troubles with the sputtering, I...
28 March 2023 3,942 4 View
Circular economy in the aviation industry.
27 March 2023 4,319 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View