Do you recommend an effective anti-OCT4 Ab for IHC?

I'm staining some brains with several stemness markers but the OCT4 antibody that I have been using is not working. I would really appreciate your recommendations! Thank you!

25 January 2021 3,169 3 View

Why does bromophenol blue band seem to split up during this SDS PAGE run?

06 January 2021 7,788 4 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View

How is possible that only a small percentage of bromophenol blue started the run in agarose electrophoresis ?

I used an 1,5% agarose gel for a second time and I loaded in the wells that I never used 15 microlitres of sample (constituted of 3 microlitres of bromophenol blue 6X, 6 microlitres of sample and...

07 December 2020 1,373 3 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How can I calculate degrees of freedom and write F for repeated measure ANOVA?

How can I calculate and report degrees of freedom for repeated mesure ANOVA? I have 48 observations N=48 and 2 factors of 3(P) and 8(LA) levels. I calculate degrees of freedom as follows: dF P =...

19 November 2020 5,682 5 View

What is the best pain killer for mice?

Good morning, I need to implant chronic electrodes in mice muscles. What is the best injectable painkiller to use during and after the surgery? and what the recommended dose for mice? And for how...

01 November 2020 8,477 1 View

Can the ramp rate of a Thermal Cycler machine influence the PCR result?

Hello, I am running PCR for genotyping. I didn't have problem till this Summer, when I couldn't see any band on the gel. I checked primers, Taq, etc. I bought new stocks etc. But still I can't...

16 September 2020 7,660 5 View

How to clean platinum/iridium single electrodes for re-use?

Hi, I will buy platinum/iridium electrodes, for single unit recording and stimulation. Since I am going to re-use them in several sessions: 1) How can I clean them? 2) Should I buy some equipment...

03 April 2018 5,828 6 View

Single electrode MICROMANIPULATOR. Which is the best?

25 March 2018 4,179 2 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

How do you obtain ASI (Annual Survey of Industries) plant identifiers, to create a panel data?

Dear all, Is there anybody who has worked with ASI (Annual Survey of Industries) Firm-Level data? I have extracted the data files with STATA, and now I need help with creating a panel for...

11 February 2021 876 4 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

Low value of Cd for Flow Past a Square Cylinder Simulation?

Hi everyone, Happy New Year! I am carrying out a Simulation for Flow Past a Square Cylinder. I am using Ansys Fluent. I get a very low value of Cd in the simulation. The solution converges but...

03 January 2021 4,389 3 View

Do we become better with vaccination from covid in 2021?

We hope to recover, and the economy improved by using vaccination .and vaccines is needed. The vaccine will protect you from getting ill and then ending up hospitalized. But it's possible that...

02 January 2021 4,194 4 View

How to add two vertical and parallel Fresnel reflection coefficients to retrieve overall reflection coefficient for a right hand circular wave?

I have a problem in which a right hand circular polarized wave (GPS wave) encounters an ideal smooth plane obliquely, and then is reflected off that. To solve this kind of problems, the wave is...

28 December 2020 2,271 3 View

How do we decide dimensions of antenna?

For standard shape (Rectangular, circular) microstrip patch antenna, standard formulas are defined for calculating dimension but for other design how do we decide shape and its dimensions? Does...

22 December 2020 5,535 3 View