Can anyone suggest some leading universities worldwide researching on nanotechnology for postdoc?

My area of research is synthesis of metal nanoparticles and their applications focusing on protein extraction from complex biological mixtures using SDS page gel electrophoresis technique. Please...

09 February 2021 2,941 3 View

How we reduce the Dyes in Botanicals like cotton pods(dry)?

For Example: If we take the botanical (Amra Pods) and we are using the dyes to give color to Amra pods . Here I take Acrylic Red(Dye). Im using 100% dye to amra pods to get it colored. Is there...

31 January 2021 1,079 1 View

List of scopus/ DOAJ indexed medical education journals?

suggest some indexed journals with low publication charges that publish medical education related research projects

22 December 2020 6,909 3 View

Stagetips for desalting small molecules (not peptides)?

I work with derivatized neurotransmitter molecules. I was using C-18 SPE cartridges previously for desalting them before LC-MS, but now I would like to adopt in-house made stagetips. These are a...

22 September 2020 4,854 6 View

What are some some helpful online sources to learn statistics, right from the basic concepts?

experiment design, data interpretation, research article writing

21 September 2020 7,723 6 View

Does the protein which serve as adjuvant can be use for vaccine candidate?

I have identified certain surface proteins which are showing both B and T cell responce and also use as adjuvant in vaccine formulation. that means we dont need any other adjuvant to induce...

14 September 2020 1,547 6 View

In the cloning process during ligation how to resolve high-ordered combinations of plasmid vector or restriction fragment?

DNA ligase only recognizes and acts on the ends of linear DNA molecules, usually result in a complex mixture of DNA molecules with randomly joined ends. The desired products (vector DNA covalently...

08 September 2020 359 2 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

16 August 2020 8,385 2 View

What is the mechanism of action of HCQ+Azi against covid 19 infection?

how H CQ+Azi acts against covid 19?

31 March 2020 4,043 9 View

How kabasura kudineer helps in covid 19 infection?

It is said that kabasura kudineer a product of Indian traditional medicine siddha/ayurveda has some beneficial effects against covid 19 infection. I would like to know more about this.

31 March 2020 1,997 21 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View