Can anyone explain how to maintain cervical cancer primary cell lines, please advise how to maintain cervical primary cell lines?

More Divya Pasumarthi's questions See All

Publish a Protocol?

Hi, please suggest some journals where I can publish a observational study protocol.

27 July 2024 2,519 0 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How to design a port or how to apply a feed for the below attached coplanar antenna?

I have designed the below attached coplanar antenna, I am unable to get the s-parameter at the required resonating frequency, so can anyone tell me how to give the proper feed?

15 May 2024 8,849 2 View

Can anyone give clear explanation add parameters for it to the parameter library first for auto dock. BEcause Ni atom is not in the library it seems?

Can anyone give clear explanation add parameters for it to the parameter library first for auto dock. Because Ni atom is not in the library it seems? Step by step instruction.

06 May 2024 4,939 1 View

Looking for journals that have fast publication and first decision time in the field of microalgal CO2 sequestration ?

Hai...could anyone suggest for me a journal with rapid publication in the field of microalgal CO2 sequestration.(review work)... a journal indexed in WOS, Scopus, SCIE with no publication...

06 May 2024 6,609 1 View

Is Pluriselect a good company to buy kits for cell separation?

I am recently buying kits from Pluriselect to take out (deplete) NK cells and CD4 cells from blood. The main reason me using this is cheap and do not require accessory equipment. But in Pubmed...

02 May 2024 5,861 1 View

I did my Ph.D in material science. Am i eligible to apply EngD?

I have submitted my Ph.D thesis and awaiting for VIVA. Am I eligible to apply EngD?

27 April 2024 1,244 3 View

What is "bonitur scale" and how to implement it in the PGP studies? Is it a good system in current research arena?

Please share the details of the bonitur scale, its significance, and its relevance in current times.

01 April 2024 2,523 0 View

How to calculate SFC value using DSC?

SFC refers to the Solid Fat Content which is generally estimated using NMR. I would like to ask about the possible ways to estimate this value using the DSC program? Please suggest.

29 March 2024 7,618 0 View

How can I calculate the SFC value for any fat using DSC thermogram instead of NMR?

SFC

15 March 2024 1,142 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View