Certain cells have reports of different expression level of SMA. Working with certain cells I have seen significant SMA expression while some other labs have not found any. Does culture conditions influence SMA expression?
The gold standard marker for SMC lineages is Myh11 (aka SM-MHC, smooth muscle myosin heavy chain). Acta2 or SMA may be induced in a variety of fibroblasts and myeloid cells as well as glial cells and stellate cells of liver. I also think it can be expressed in mesagial cells of kidney under stress conditions. There is quite a literature on this subject. My question relates to Brian's : what exactly are you trying to do?
Hi Sandipto,
Substrate stiffness is a crucial factor regulating SMA expression. Similarly, factors that may influence cell fate and differentiation affect SMA levels.
Another possibility would be that your to their cells are not what they are supposed to be.
This is all I can tell you based on the very limited info you have provided me with.
Best,
Metello
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