Joseph Miano

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Questions related from Joseph Miano

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

24 February 2021 8,802 3 View

What conditions and anti-FLAG antibody are proven to work in tissue sections of mouse?

We recently made a FLAG-tagged mouse using 3 component CRISPR. We can do ChIP and Westerns very well. However, detecting the FLAG epitope in mouse sections (either frozen or paraffin) have been...

01 January 1970 6,537 4 View