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Questions related from Joseph Miano
Has anyone had success with tamoxifen (or 4-hydroxytamoxifen) inducible Cre drivers in the rat? We heard of cases where rats were killed with high doses Tmx (>100 mg/kg). Even at 20 mg/kg we...
20 June 2023 1,662 0 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
24 February 2021 8,825 3 View
The NgAgo nuclease uses a 5' phosphorylated single-strand DNA guide to create a double strand break in genomic DNA. It has been proposed as a superior genome editing system over CRISPR-Cas9....
14 August 2016 2,330 5 View
The these assays utilize methods of "tagging" DSBs in the genome followed by PCR amplification of the library of sequences and NGS. They both seem rather temperamental but I wonder who (beyond...
09 July 2015 2,336 0 View
We recently made a FLAG-tagged mouse using 3 component CRISPR. We can do ChIP and Westerns very well. However, detecting the FLAG epitope in mouse sections (either frozen or paraffin) have been...
01 January 1970 6,547 4 View