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Questions related from Joseph Miano
Has anyone had success with tamoxifen (or 4-hydroxytamoxifen) inducible Cre drivers in the rat? We heard of cases where rats were killed with high doses Tmx (>100 mg/kg). Even at 20 mg/kg we...
20 June 2023 1,716 0 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
24 February 2021 9,001 3 View
I need a BAC that spans the entire human FLI1 locus. There is one annotated on the UCSC genome browser but, as bad luck would have it, this clone is no longer available. How can I get a BAC That...
21 March 2017 5,299 3 View
The NgAgo nuclease uses a 5' phosphorylated single-strand DNA guide to create a double strand break in genomic DNA. It has been proposed as a superior genome editing system over CRISPR-Cas9....
14 August 2016 2,382 5 View
The these assays utilize methods of "tagging" DSBs in the genome followed by PCR amplification of the library of sequences and NGS. They both seem rather temperamental but I wonder who (beyond...
09 July 2015 2,380 0 View
We recently made a FLAG-tagged mouse using 3 component CRISPR. We can do ChIP and Westerns very well. However, detecting the FLAG epitope in mouse sections (either frozen or paraffin) have been...
01 January 1970 6,587 4 View