Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

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Anu Makela

Histone H3 K4Me3 antibody Description: Rabbit polyclonal antibody recognizing the histone H3 trimethylated at lysine 4.

Histone H3 K4Me3 Antibody for use in ChIP / ChIP-seq (4-10 µl per reaction), immunofluorescence (1:500 dilution) and Western blot (1:1,000 - 1:3,500).

From EpiCypher, catalog number 13-0004

http://www.epicypher.com/antibodies.aspx

Sorry, added too early...

Other Antibodies, their working dilutions and their supplier are as follows: monoclonal p220NPAT (mouse monoclonal, 1:1000, BD Biosciences), polyclonal p220NPAT (rabbit polyclonal, 1:1000) (Ma et al, 2000; Zhao et al, 2000), coilin (pdelta) (mouse monoclonal, 1:500, Santa Cruz Biotech), coilin (rabbit polyclonal, 1:500, (Andrade et al, 1991)) and Lsm10 (mouse monoclonal, 1:500, Biomatrix Research). Secondary antibodies were goat anti-mouse Alexa 488, goat anti-rabbit Alexa 488, goat anti-mouse Alexa 594, goat anti-rabbit Alexa 594, goat anti-mouse Alexa 350, goat anti-rabbit Alexa 350 (all 1:800 dilution in 1X PBSA).

Antoine Zalc

Hey,

You can try www.benchwise.org.

The Crabtree's lab shared all their antibody database with a lot of histone antibodies.

Protocols are also available (http://www.benchwise.org/50cc2176a6567702000003dc)

Hope you'll find anything you need !