How reliable are degrees of freedom for lme mixed models in nmle package?

I am running mixed models analyses because I have participants nested in groups. Across the different analyses, I have very different degrees of freedom in the lme output with nlme package (and...

09 February 2021 6,767 3 View

What is the best visualisation solvent for xylooligosaccharides using TLC?

I am interested in using TLC to detect non-linear xylooligosaccharides. What would be the best visualisation solvent? Would aniline phthalate solution be suitable? I have also seen people...

02 February 2021 7,748 3 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

How to measure CPT1/FAO activity in a rare cell type?

Hello all, I am searching for a way to measure the activity of CPT1 and/or FAO in mouse hematopoietic stem cells, which means I have very little material available to do so. Any...

20 January 2021 8,499 2 View

Power based on past research in multilevel model?

Hi I want to replicate a published article that uses a multilevel model (participants nested into groups). I want to see the sample size I need to achieve an 80% power. I saw the simr r package...

06 January 2021 2,984 6 View

Can I display regression line for a whole group, when subgroups are displayed on Graphpad Prism?

I have created a scatter graph in Prism under -> multivariable analyses -> extract and re-arrange, to display relationship between 2 variables, the 3 subgroups within the group are...

16 October 2020 310 1 View

I am writing a literature review on negative symptoms and how cognitive neuroscience has she light on the understanding of schizophrenia?

This is my first time to write a literature review. I would appreciate some tips and suggestions. Links to good examples or informative websites. Papers on the topic.

13 September 2020 4,141 5 View

Are there any RNA secondary structure prediction programs for long sequences?

Can anyone help please. I am looking for a free, and preferably web based, program that will predict the secondary structure of a long RNA sequence. The most common programs only accept short...

07 July 2020 1,880 3 View

Survey Data Anlysis/Testing Support - any help?

Hi - hoping someone might be able to help me please? I've had to totally change my MSc project as a result of COVID and as I can no longer do face to face testing, I have created a survey and...

14 April 2020 6,292 9 View

What's the best way to administer or score measures of overclaiming?

I'm looking for advice from folks familiar with the research on overclaiming, social desirability or lying, or general measurement expertise, on ways of administering or scoring the overclaiming...

29 January 2020 7,520 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View