when I blast the existed primers specially 16s rDNAs , I cant get good results.
Primer 27f/1492r is the most widely used primer for species-level identification. Currently available primers can reveal the composition of the predominant bacteria in a sample through amplification and sequencing.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127287/
Kindly check https://academic.oup.com/femsle/article/214/2/271/611104
Also check https://aem.asm.org/content/68/1/114
Thank you so much
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