These are for cells and hopefully be retained in the cells after fixation.
I need a small volume for 1-2 experiments and cannot afford to spend too much money over it!
Can you tell us a little more about what you want to do with this? Which organelle do you want to label? Does it necessarily need to be by immunolabeling or would a transfection with a fluorescent-marker do, too?
Thanks.
Hi Joaquin,
I need these for some colocalization experiments. ER and Golgi are what I want the markers for. I already have the fluorescent markers for transfection but am not very happy with the health of the cells after transfection. Since I already know that Mitotracker dyes work beautifully for mitochondria, I was thinking of trying similar fluorescent probes for other organelles.
Thermo Fisher has a range of these but they are quite expensive. So, I wanted to know if anyone knows of a company which would give small free samples or even small sample sizes for a reduced price!
Cell Signaling offers Trial Sizes of their antibodies.Santa Cruz will give you free antibodies if you cite them. The other possibility you have is to call the company that offers the antibody you want (and know it works) and ask to speak with the sales representative. Most companies offer free evaluation samples. I guess that would be the cheapest way.
Hope this helps!
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