How to model the vapor plume during laser welding process using Fluent?

Problem description: I am using ANSYS Fluent (multiphase VOF & solidification and melting model) to simulate laser welding process. Is there any possibility of establishing a fully coupled...

05 November 2020 893 1 View

Should I add penicillin/streptomycin into cell culture media for doxycycline inducible system?

Hello, since I have no experience in Tet-on system in drosophila S2 cell culture, I need some technical help... Should I add P/S into the cell culture media? What's the doxycycline concentration...

17 September 2020 7,497 5 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View

Black paint for supercapacitor electrodes?

Do anyone knows what kind of materials could be used to paint a supercapacitor electrode as shown in the picture (the coated black shining materials)? Any help is appreciated. Thanks!

05 March 2020 2,194 4 View

Which short versions of Ryff's Psychological Well-Being Scale do you recommend?

I want to assess the well-being of farmers in Southeast through both an assets and psychological perspective and am having trouble finding a good measurement scale for the latter. Ideally, I want...

03 January 2020 1,927 3 View

What type of brain cell or tissue is this?

We recently discovered a probe could stain brain tissues and showed a very specific distribution, wondering what type of cells or tissue is this?

03 March 2019 7,214 6 View

Anyone who can help me provied some Corresponding Codes on Active Learning?

Anyone who can help me provied some Corresponding Codes on Active Learning? such as the batch-model active learning. Matlab codes are the best. Something else on the knowledge of AL is also useful...

16 February 2019 8,512 4 View

Hey, does anybody know if there are some bacteria can use acetate to produce methanol or methylamine?

any bacteria can use acetate to produce methanol or methylamine? or use H2 and CO2 to produce methanol?

15 May 2018 9,401 5 View

Any one who is working on or interested in vision problems on videos?

I'm very happy to learn, discuss and collaborate on some vision topics on videos. If you are also very interested, feel free to contact me.

10 May 2018 4,869 2 View

In Abaqus what is the most efficient way to add wire feature

08 July 2017 8,539 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View