Should I add penicillin/streptomycin into cell culture media for doxycycline inducible system?

Hello, since I have no experience in Tet-on system in drosophila S2 cell culture, I need some technical help... Should I add P/S into the cell culture media? What's the doxycycline concentration...

17 September 2020 7,497 5 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View

Black paint for supercapacitor electrodes?

Do anyone knows what kind of materials could be used to paint a supercapacitor electrode as shown in the picture (the coated black shining materials)? Any help is appreciated. Thanks!

05 March 2020 2,194 4 View

Which short versions of Ryff's Psychological Well-Being Scale do you recommend?

I want to assess the well-being of farmers in Southeast through both an assets and psychological perspective and am having trouble finding a good measurement scale for the latter. Ideally, I want...

03 January 2020 1,927 3 View

What type of brain cell or tissue is this?

We recently discovered a probe could stain brain tissues and showed a very specific distribution, wondering what type of cells or tissue is this?

03 March 2019 7,214 6 View

Hey, does anybody know if there are some bacteria can use acetate to produce methanol or methylamine?

any bacteria can use acetate to produce methanol or methylamine? or use H2 and CO2 to produce methanol?

15 May 2018 9,401 5 View

Any one who is working on or interested in vision problems on videos?

I'm very happy to learn, discuss and collaborate on some vision topics on videos. If you are also very interested, feel free to contact me.

10 May 2018 4,869 2 View

In Abaqus what is the most efficient way to add wire feature

08 July 2017 8,539 5 View

T lymphocyte apoptosis in mice

We have found that deleting a transcription factor in Tregs causes fatal autoimmune disorder in mice, concomitant with accumulation of effector memory CD4 cells (CD44hi CD62L low) in blood, LN and...

01 July 2017 4,423 1 View

Can anybody tell the difference between BMDM and immortalized BMDM cells?

06 June 2017 6,522 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

How to extract the y+ value from fluent ?

I am doing a 3D steady simulation of a wind rotor using Ansys Fluent. In order to have a y+ of 1, I created 10 inflation layers using total layer thickness method, and I started a first...

23 February 2021 2,819 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View