We recently discovered a probe could stain brain tissues and showed a very specific distribution, wondering what type of cells or tissue is this?
Hi, in IMG12 you clearly see the hippocampal region Dentate Gyrus (DG), CA3, CA1 as indicated by stained Granule Cell in the DG forming this V-like structure, and starting from here, CA-pyramidal neurons forming CA4, CA3, CA1 as a C-like structure. Thus, you stained hippocampal neurons in dark blue.
The same hold true for IMG13, but now those hippocampal neurons are stained in purple. Again, you easily recon the V-like DG region and the C-like Cornu Ammonis (CA) regions. And in the lower left corner, below you hippocampus, you see stained neurons of the neocortex also in purple.
Is that rodent-tissue?
I hope this will help you. Feel free to ask further questions....
Best, Ricardo
Hy,
it is surely rat or mouse brain tissue but due to the fact that there is no scale bar it is not easy to clarify.
I cannot recognize a cell staining but a nice nerve-fiber or myelin staining (for example in the corpus callosum of the first Picture or in the last one).
Perhaps your probe stains neurofilaments or microtubules.
I hope this helps you
KInd regards
Stefan
This looks very non-specific, to my eyes. If you provide more information about how the images were acquired (epi-fluorescence? excitation and emission filters? exposure?) maybe we could say more. If anything, it seems like neuropil is stained more than cell bodies.
You should also take images of an unstained, neighbouring slice, and image both with same parameters. The "unstained" slice should undergo all the same processing (washes, incubation times, etc) as the stained slice, except leave out the actual stain. Then show side-by-side. Differences will indicate any specific staining.
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